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heat shock protein 90α levels  (Cusabio)


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    Cusabio heat shock protein 90α levels
    Cell viabilities of (A) H69, (B) H82, and (C) H146 cells under the treatment of ABT-737 or doxorubicin. Enzyme-linked immunosorbent assay (ELISA) assay revealed the existence and concentrations of extracellular heat shock protein <t>90α</t> (HSP 90α) in the medium of (D) H69, (E) H82, and (F) H146 cells under the treatment of ABT-737 or doxorubicin; (G) representative and (H) summary of Western blot assay for the existence and quantities of HSP 90α and β in culture medium of various cell types (as in eHSP90α and eHSP90β), and the amount of intracellular HSP 90α and β. * P < .05, ** P = .01, *** P = .001, and † P > .05, respectively, compared to control. # P < .05, ## P = .01, ### P = .001, and ‡ P > .05, respectively, compared to models or indicated.
    Heat Shock Protein 90α Levels, supplied by Cusabio, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/heat+shock+protein+90%CE%B1+levels/Human+heat+shock+protein+90%CE%B1+(HSP-90%CE%B1)+ELISA+kit/pmc06172942-33-0-13
    Average 88 stars, based on 2 article reviews
    heat shock protein 90α levels - by Bioz Stars, 2026-10
    88/100 stars

    Images

    1) Product Images from "Secreted Heat Shock Protein 90α Attenuated the Effect of Anticancer Drugs in Small-Cell Lung Cancer Cells Through AKT/GSK3β/β-Catenin Signaling"

    Article Title: Secreted Heat Shock Protein 90α Attenuated the Effect of Anticancer Drugs in Small-Cell Lung Cancer Cells Through AKT/GSK3β/β-Catenin Signaling

    Journal: Cancer Control : Journal of the Moffitt Cancer Center

    doi: 10.1177/1073274818804489

    Cell viabilities of (A) H69, (B) H82, and (C) H146 cells under the treatment of ABT-737 or doxorubicin. Enzyme-linked immunosorbent assay (ELISA) assay revealed the existence and concentrations of extracellular heat shock protein 90α (HSP 90α) in the medium of (D) H69, (E) H82, and (F) H146 cells under the treatment of ABT-737 or doxorubicin; (G) representative and (H) summary of Western blot assay for the existence and quantities of HSP 90α and β in culture medium of various cell types (as in eHSP90α and eHSP90β), and the amount of intracellular HSP 90α and β. * P < .05, ** P = .01, *** P = .001, and † P > .05, respectively, compared to control. # P < .05, ## P = .01, ### P = .001, and ‡ P > .05, respectively, compared to models or indicated.
    Figure Legend Snippet: Cell viabilities of (A) H69, (B) H82, and (C) H146 cells under the treatment of ABT-737 or doxorubicin. Enzyme-linked immunosorbent assay (ELISA) assay revealed the existence and concentrations of extracellular heat shock protein 90α (HSP 90α) in the medium of (D) H69, (E) H82, and (F) H146 cells under the treatment of ABT-737 or doxorubicin; (G) representative and (H) summary of Western blot assay for the existence and quantities of HSP 90α and β in culture medium of various cell types (as in eHSP90α and eHSP90β), and the amount of intracellular HSP 90α and β. * P < .05, ** P = .01, *** P = .001, and † P > .05, respectively, compared to control. # P < .05, ## P = .01, ### P = .001, and ‡ P > .05, respectively, compared to models or indicated.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Western Blot, Control

    Apoptosis of cells after different treatments were tested and presented as (A) representative and (B-G) summaries of cell survival rates. Concentrations of human recombinant heat shock protein 90 (hrHSP90) were 10 and 40 μg/mL. * P < .05, ** P = .01, *** P = .001, and † P > .05, respectively, compared to control. # P < .05, ## P = .01, ### P = .001, and ‡ P > .05, respectively, compared to models or indicated.
    Figure Legend Snippet: Apoptosis of cells after different treatments were tested and presented as (A) representative and (B-G) summaries of cell survival rates. Concentrations of human recombinant heat shock protein 90 (hrHSP90) were 10 and 40 μg/mL. * P < .05, ** P = .01, *** P = .001, and † P > .05, respectively, compared to control. # P < .05, ## P = .01, ### P = .001, and ‡ P > .05, respectively, compared to models or indicated.

    Techniques Used: Recombinant, Control

    Extracellular heat shock protein 90α (HSP90α) inhibits glycogen synthase kinase 3β (GSK3β) via the activation of Ak strain transforming (AKT) signaling. A, Western blot assay of phosphorylation of AKT (p-AKT; T308), p-AKT (S473), AKT, phosphorylation of GSK3β (p-GSK3β) and GSK3β in H69, H82, and H146 cells after 40 μg/mL human recombinant heat shock protein 90 (hrHSP90) treatment. B, Summaries of p-AKT (308) and AKT levels in (A). C, Summaries of p-AKT(S473) levels in (A). D, Summaries of p-GSK3β and GSK3β levels in (A). * P < .05, ** P = .01, *** P = .001, and † P > .05, respectively, compared to control. # P < .05, ## P = .01, ### P = .001, and ‡ P > .05, respectively, compared to models or indicated.
    Figure Legend Snippet: Extracellular heat shock protein 90α (HSP90α) inhibits glycogen synthase kinase 3β (GSK3β) via the activation of Ak strain transforming (AKT) signaling. A, Western blot assay of phosphorylation of AKT (p-AKT; T308), p-AKT (S473), AKT, phosphorylation of GSK3β (p-GSK3β) and GSK3β in H69, H82, and H146 cells after 40 μg/mL human recombinant heat shock protein 90 (hrHSP90) treatment. B, Summaries of p-AKT (308) and AKT levels in (A). C, Summaries of p-AKT(S473) levels in (A). D, Summaries of p-GSK3β and GSK3β levels in (A). * P < .05, ** P = .01, *** P = .001, and † P > .05, respectively, compared to control. # P < .05, ## P = .01, ### P = .001, and ‡ P > .05, respectively, compared to models or indicated.

    Techniques Used: Activation Assay, Western Blot, Phospho-proteomics, Recombinant, Control

    Subsequently after the inhibition of glycogen synthase kinase 3β (GSK3β), extracellular heat shock protein 90α (HSP90α) could then activate β-catenin. A, Western blot assay of active β-catenin and β-catenin in H69, H82, and H146 cells after 40 μg/mL human recombinant heat shock protein 90 (hrHSP90) treatment. (B) Summaries of active β-catenin and β-catenin levels in A. (C) Akt inhibitor MK-2206 could partly reverse the phosphorylation states of various proteins after hrHSP90 treatment. * P < .05, ** P = .01, *** P = .001, and † P > .05, respectively, compared to control. # P < .05, ## P = .01, ### P = .001, and ‡ P > .05, respectively, compared to models or indicated.
    Figure Legend Snippet: Subsequently after the inhibition of glycogen synthase kinase 3β (GSK3β), extracellular heat shock protein 90α (HSP90α) could then activate β-catenin. A, Western blot assay of active β-catenin and β-catenin in H69, H82, and H146 cells after 40 μg/mL human recombinant heat shock protein 90 (hrHSP90) treatment. (B) Summaries of active β-catenin and β-catenin levels in A. (C) Akt inhibitor MK-2206 could partly reverse the phosphorylation states of various proteins after hrHSP90 treatment. * P < .05, ** P = .01, *** P = .001, and † P > .05, respectively, compared to control. # P < .05, ## P = .01, ### P = .001, and ‡ P > .05, respectively, compared to models or indicated.

    Techniques Used: Inhibition, Western Blot, Recombinant, Phospho-proteomics, Control

    Extracellular heat shock protein 90α (HSP90α) but not HSP90β increased the phosphorylation levels of Ak strain transforming (AKT) and glycogen synthase kinase 3β (GSK3β) while enhanced the expression of active β-catenin. (A) Tumor volumes of xenograft mouse after various treatments. (B) Representative and (C) summary of the phosphorylation and expression levels in tumor areas. (D) Representative and (E) summary of the transferase dUTP nick end labeling (TUNEL) staining indicating the apoptosis of tumor cells under different treatment. * P < .05, ** P = .01, *** P = .001, and † P > .05, respectively, compared to control. # P < .05, ## P = .01, ### P = .001, and ‡ P > .05, respectively, compared to models or indicated.
    Figure Legend Snippet: Extracellular heat shock protein 90α (HSP90α) but not HSP90β increased the phosphorylation levels of Ak strain transforming (AKT) and glycogen synthase kinase 3β (GSK3β) while enhanced the expression of active β-catenin. (A) Tumor volumes of xenograft mouse after various treatments. (B) Representative and (C) summary of the phosphorylation and expression levels in tumor areas. (D) Representative and (E) summary of the transferase dUTP nick end labeling (TUNEL) staining indicating the apoptosis of tumor cells under different treatment. * P < .05, ** P = .01, *** P = .001, and † P > .05, respectively, compared to control. # P < .05, ## P = .01, ### P = .001, and ‡ P > .05, respectively, compared to models or indicated.

    Techniques Used: Phospho-proteomics, Expressing, End Labeling, TUNEL Assay, Staining, Control

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Secreted Heat Shock Protein 90α Attenuated the Effect of Anticancer Drugs in Small-Cell Lung Cancer Cells Through AKT/GSK3β/β-Catenin Signaling
    Article Snippet: Then, the medium was collected, followed by removing debris with centrifugation (5 minutes, 1200 g ).Then, the medium was collected, followed by removing debris with centrifugation (5 minutes, 1200 g ).. Heat shock protein 90α levels were detected using Hsp90α enzyme-linked immunosorbent assay kit (Cusabio, CSB-E13462 h, Houston, Texas) according to the manufacturer’s instructions.. All detections were performed at least 3 times.All detections were performed at least 3 times.

    Western Blot:

    Article Title: Secreted Heat Shock Protein 90α Attenuated the Effect of Anticancer Drugs in Small-Cell Lung Cancer Cells Through AKT/GSK3β/β-Catenin Signaling
    Article Snippet: Then, the medium was collected, followed by removing debris with centrifugation (5 minutes, 1200 g ).Then, the medium was collected, followed by removing debris with centrifugation (5 minutes, 1200 g ).. Heat shock protein 90α levels were detected using Hsp90α enzyme-linked immunosorbent assay kit (Cusabio, CSB-E13462 h, Houston, Texas) according to the manufacturer’s instructions.. All detections were performed at least 3 times.All detections were performed at least 3 times.

    Control:

    Article Title: Secreted Heat Shock Protein 90α Attenuated the Effect of Anticancer Drugs in Small-Cell Lung Cancer Cells Through AKT/GSK3β/β-Catenin Signaling
    Article Snippet: Then, the medium was collected, followed by removing debris with centrifugation (5 minutes, 1200 g ).Then, the medium was collected, followed by removing debris with centrifugation (5 minutes, 1200 g ).. Heat shock protein 90α levels were detected using Hsp90α enzyme-linked immunosorbent assay kit (Cusabio, CSB-E13462 h, Houston, Texas) according to the manufacturer’s instructions.. All detections were performed at least 3 times.All detections were performed at least 3 times.

    Recombinant:

    Article Title: Secreted Heat Shock Protein 90α Attenuated the Effect of Anticancer Drugs in Small-Cell Lung Cancer Cells Through AKT/GSK3β/β-Catenin Signaling
    Article Snippet: Then, the medium was collected, followed by removing debris with centrifugation (5 minutes, 1200 g ).Then, the medium was collected, followed by removing debris with centrifugation (5 minutes, 1200 g ).. Heat shock protein 90α levels were detected using Hsp90α enzyme-linked immunosorbent assay kit (Cusabio, CSB-E13462 h, Houston, Texas) according to the manufacturer’s instructions.. All detections were performed at least 3 times.All detections were performed at least 3 times.

    Activation Assay:

    Article Title: Secreted Heat Shock Protein 90α Attenuated the Effect of Anticancer Drugs in Small-Cell Lung Cancer Cells Through AKT/GSK3β/β-Catenin Signaling
    Article Snippet: Then, the medium was collected, followed by removing debris with centrifugation (5 minutes, 1200 g ).Then, the medium was collected, followed by removing debris with centrifugation (5 minutes, 1200 g ).. Heat shock protein 90α levels were detected using Hsp90α enzyme-linked immunosorbent assay kit (Cusabio, CSB-E13462 h, Houston, Texas) according to the manufacturer’s instructions.. All detections were performed at least 3 times.All detections were performed at least 3 times.

    Phospho-proteomics:

    Article Title: Secreted Heat Shock Protein 90α Attenuated the Effect of Anticancer Drugs in Small-Cell Lung Cancer Cells Through AKT/GSK3β/β-Catenin Signaling
    Article Snippet: Then, the medium was collected, followed by removing debris with centrifugation (5 minutes, 1200 g ).Then, the medium was collected, followed by removing debris with centrifugation (5 minutes, 1200 g ).. Heat shock protein 90α levels were detected using Hsp90α enzyme-linked immunosorbent assay kit (Cusabio, CSB-E13462 h, Houston, Texas) according to the manufacturer’s instructions.. All detections were performed at least 3 times.All detections were performed at least 3 times.

    Inhibition:

    Article Title: Secreted Heat Shock Protein 90α Attenuated the Effect of Anticancer Drugs in Small-Cell Lung Cancer Cells Through AKT/GSK3β/β-Catenin Signaling
    Article Snippet: Then, the medium was collected, followed by removing debris with centrifugation (5 minutes, 1200 g ).Then, the medium was collected, followed by removing debris with centrifugation (5 minutes, 1200 g ).. Heat shock protein 90α levels were detected using Hsp90α enzyme-linked immunosorbent assay kit (Cusabio, CSB-E13462 h, Houston, Texas) according to the manufacturer’s instructions.. All detections were performed at least 3 times.All detections were performed at least 3 times.

    Expressing:

    Article Title: Secreted Heat Shock Protein 90α Attenuated the Effect of Anticancer Drugs in Small-Cell Lung Cancer Cells Through AKT/GSK3β/β-Catenin Signaling
    Article Snippet: Then, the medium was collected, followed by removing debris with centrifugation (5 minutes, 1200 g ).Then, the medium was collected, followed by removing debris with centrifugation (5 minutes, 1200 g ).. Heat shock protein 90α levels were detected using Hsp90α enzyme-linked immunosorbent assay kit (Cusabio, CSB-E13462 h, Houston, Texas) according to the manufacturer’s instructions.. All detections were performed at least 3 times.All detections were performed at least 3 times.

    End Labeling:

    Article Title: Secreted Heat Shock Protein 90α Attenuated the Effect of Anticancer Drugs in Small-Cell Lung Cancer Cells Through AKT/GSK3β/β-Catenin Signaling
    Article Snippet: Then, the medium was collected, followed by removing debris with centrifugation (5 minutes, 1200 g ).Then, the medium was collected, followed by removing debris with centrifugation (5 minutes, 1200 g ).. Heat shock protein 90α levels were detected using Hsp90α enzyme-linked immunosorbent assay kit (Cusabio, CSB-E13462 h, Houston, Texas) according to the manufacturer’s instructions.. All detections were performed at least 3 times.All detections were performed at least 3 times.

    TUNEL Assay:

    Article Title: Secreted Heat Shock Protein 90α Attenuated the Effect of Anticancer Drugs in Small-Cell Lung Cancer Cells Through AKT/GSK3β/β-Catenin Signaling
    Article Snippet: Then, the medium was collected, followed by removing debris with centrifugation (5 minutes, 1200 g ).Then, the medium was collected, followed by removing debris with centrifugation (5 minutes, 1200 g ).. Heat shock protein 90α levels were detected using Hsp90α enzyme-linked immunosorbent assay kit (Cusabio, CSB-E13462 h, Houston, Texas) according to the manufacturer’s instructions.. All detections were performed at least 3 times.All detections were performed at least 3 times.

    Staining:

    Article Title: Secreted Heat Shock Protein 90α Attenuated the Effect of Anticancer Drugs in Small-Cell Lung Cancer Cells Through AKT/GSK3β/β-Catenin Signaling
    Article Snippet: Then, the medium was collected, followed by removing debris with centrifugation (5 minutes, 1200 g ).Then, the medium was collected, followed by removing debris with centrifugation (5 minutes, 1200 g ).. Heat shock protein 90α levels were detected using Hsp90α enzyme-linked immunosorbent assay kit (Cusabio, CSB-E13462 h, Houston, Texas) according to the manufacturer’s instructions.. All detections were performed at least 3 times.All detections were performed at least 3 times.



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    heat shock protein 90α levels  (Cusabio)
    88
    Cusabio heat shock protein 90α levels
    Cell viabilities of (A) H69, (B) H82, and (C) H146 cells under the treatment of ABT-737 or doxorubicin. Enzyme-linked immunosorbent assay (ELISA) assay revealed the existence and concentrations of extracellular heat shock protein <t>90α</t> (HSP 90α) in the medium of (D) H69, (E) H82, and (F) H146 cells under the treatment of ABT-737 or doxorubicin; (G) representative and (H) summary of Western blot assay for the existence and quantities of HSP 90α and β in culture medium of various cell types (as in eHSP90α and eHSP90β), and the amount of intracellular HSP 90α and β. * P < .05, ** P = .01, *** P = .001, and † P > .05, respectively, compared to control. # P < .05, ## P = .01, ### P = .001, and ‡ P > .05, respectively, compared to models or indicated.
    Heat Shock Protein 90α Levels, supplied by Cusabio, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/heat+shock+protein+90%CE%B1+levels/Human+heat+shock+protein+90%CE%B1+(HSP-90%CE%B1)+ELISA+kit/pmc06172942-33-0-13
    Average 88 stars, based on 1 article reviews
    heat shock protein 90α levels - by Bioz Stars, 2026-10
    88/100 stars
    		  Buy from Supplier

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    Cell viabilities of (A) H69, (B) H82, and (C) H146 cells under the treatment of ABT-737 or doxorubicin. Enzyme-linked immunosorbent assay (ELISA) assay revealed the existence and concentrations of extracellular heat shock protein 90α (HSP 90α) in the medium of (D) H69, (E) H82, and (F) H146 cells under the treatment of ABT-737 or doxorubicin; (G) representative and (H) summary of Western blot assay for the existence and quantities of HSP 90α and β in culture medium of various cell types (as in eHSP90α and eHSP90β), and the amount of intracellular HSP 90α and β. * P < .05, ** P = .01, *** P = .001, and † P > .05, respectively, compared to control. # P < .05, ## P = .01, ### P = .001, and ‡ P > .05, respectively, compared to models or indicated.

    Journal: Cancer Control : Journal of the Moffitt Cancer Center

    Article Title: Secreted Heat Shock Protein 90α Attenuated the Effect of Anticancer Drugs in Small-Cell Lung Cancer Cells Through AKT/GSK3β/β-Catenin Signaling

    doi: 10.1177/1073274818804489

    Figure Lengend Snippet: Cell viabilities of (A) H69, (B) H82, and (C) H146 cells under the treatment of ABT-737 or doxorubicin. Enzyme-linked immunosorbent assay (ELISA) assay revealed the existence and concentrations of extracellular heat shock protein 90α (HSP 90α) in the medium of (D) H69, (E) H82, and (F) H146 cells under the treatment of ABT-737 or doxorubicin; (G) representative and (H) summary of Western blot assay for the existence and quantities of HSP 90α and β in culture medium of various cell types (as in eHSP90α and eHSP90β), and the amount of intracellular HSP 90α and β. * P < .05, ** P = .01, *** P = .001, and † P > .05, respectively, compared to control. # P < .05, ## P = .01, ### P = .001, and ‡ P > .05, respectively, compared to models or indicated.

    Article Snippet: Heat shock protein 90α levels were detected using Hsp90α enzyme-linked immunosorbent assay kit (Cusabio, CSB-E13462 h, Houston, Texas) according to the manufacturer’s instructions.

    Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Control

    Apoptosis of cells after different treatments were tested and presented as (A) representative and (B-G) summaries of cell survival rates. Concentrations of human recombinant heat shock protein 90 (hrHSP90) were 10 and 40 μg/mL. * P < .05, ** P = .01, *** P = .001, and † P > .05, respectively, compared to control. # P < .05, ## P = .01, ### P = .001, and ‡ P > .05, respectively, compared to models or indicated.

    Journal: Cancer Control : Journal of the Moffitt Cancer Center

    Article Title: Secreted Heat Shock Protein 90α Attenuated the Effect of Anticancer Drugs in Small-Cell Lung Cancer Cells Through AKT/GSK3β/β-Catenin Signaling

    doi: 10.1177/1073274818804489

    Figure Lengend Snippet: Apoptosis of cells after different treatments were tested and presented as (A) representative and (B-G) summaries of cell survival rates. Concentrations of human recombinant heat shock protein 90 (hrHSP90) were 10 and 40 μg/mL. * P < .05, ** P = .01, *** P = .001, and † P > .05, respectively, compared to control. # P < .05, ## P = .01, ### P = .001, and ‡ P > .05, respectively, compared to models or indicated.

    Article Snippet: Heat shock protein 90α levels were detected using Hsp90α enzyme-linked immunosorbent assay kit (Cusabio, CSB-E13462 h, Houston, Texas) according to the manufacturer’s instructions.

    Techniques: Recombinant, Control

    Extracellular heat shock protein 90α (HSP90α) inhibits glycogen synthase kinase 3β (GSK3β) via the activation of Ak strain transforming (AKT) signaling. A, Western blot assay of phosphorylation of AKT (p-AKT; T308), p-AKT (S473), AKT, phosphorylation of GSK3β (p-GSK3β) and GSK3β in H69, H82, and H146 cells after 40 μg/mL human recombinant heat shock protein 90 (hrHSP90) treatment. B, Summaries of p-AKT (308) and AKT levels in (A). C, Summaries of p-AKT(S473) levels in (A). D, Summaries of p-GSK3β and GSK3β levels in (A). * P < .05, ** P = .01, *** P = .001, and † P > .05, respectively, compared to control. # P < .05, ## P = .01, ### P = .001, and ‡ P > .05, respectively, compared to models or indicated.

    Journal: Cancer Control : Journal of the Moffitt Cancer Center

    Article Title: Secreted Heat Shock Protein 90α Attenuated the Effect of Anticancer Drugs in Small-Cell Lung Cancer Cells Through AKT/GSK3β/β-Catenin Signaling

    doi: 10.1177/1073274818804489

    Figure Lengend Snippet: Extracellular heat shock protein 90α (HSP90α) inhibits glycogen synthase kinase 3β (GSK3β) via the activation of Ak strain transforming (AKT) signaling. A, Western blot assay of phosphorylation of AKT (p-AKT; T308), p-AKT (S473), AKT, phosphorylation of GSK3β (p-GSK3β) and GSK3β in H69, H82, and H146 cells after 40 μg/mL human recombinant heat shock protein 90 (hrHSP90) treatment. B, Summaries of p-AKT (308) and AKT levels in (A). C, Summaries of p-AKT(S473) levels in (A). D, Summaries of p-GSK3β and GSK3β levels in (A). * P < .05, ** P = .01, *** P = .001, and † P > .05, respectively, compared to control. # P < .05, ## P = .01, ### P = .001, and ‡ P > .05, respectively, compared to models or indicated.

    Article Snippet: Heat shock protein 90α levels were detected using Hsp90α enzyme-linked immunosorbent assay kit (Cusabio, CSB-E13462 h, Houston, Texas) according to the manufacturer’s instructions.

    Techniques: Activation Assay, Western Blot, Phospho-proteomics, Recombinant, Control

    Subsequently after the inhibition of glycogen synthase kinase 3β (GSK3β), extracellular heat shock protein 90α (HSP90α) could then activate β-catenin. A, Western blot assay of active β-catenin and β-catenin in H69, H82, and H146 cells after 40 μg/mL human recombinant heat shock protein 90 (hrHSP90) treatment. (B) Summaries of active β-catenin and β-catenin levels in A. (C) Akt inhibitor MK-2206 could partly reverse the phosphorylation states of various proteins after hrHSP90 treatment. * P < .05, ** P = .01, *** P = .001, and † P > .05, respectively, compared to control. # P < .05, ## P = .01, ### P = .001, and ‡ P > .05, respectively, compared to models or indicated.

    Journal: Cancer Control : Journal of the Moffitt Cancer Center

    Article Title: Secreted Heat Shock Protein 90α Attenuated the Effect of Anticancer Drugs in Small-Cell Lung Cancer Cells Through AKT/GSK3β/β-Catenin Signaling

    doi: 10.1177/1073274818804489

    Figure Lengend Snippet: Subsequently after the inhibition of glycogen synthase kinase 3β (GSK3β), extracellular heat shock protein 90α (HSP90α) could then activate β-catenin. A, Western blot assay of active β-catenin and β-catenin in H69, H82, and H146 cells after 40 μg/mL human recombinant heat shock protein 90 (hrHSP90) treatment. (B) Summaries of active β-catenin and β-catenin levels in A. (C) Akt inhibitor MK-2206 could partly reverse the phosphorylation states of various proteins after hrHSP90 treatment. * P < .05, ** P = .01, *** P = .001, and † P > .05, respectively, compared to control. # P < .05, ## P = .01, ### P = .001, and ‡ P > .05, respectively, compared to models or indicated.

    Article Snippet: Heat shock protein 90α levels were detected using Hsp90α enzyme-linked immunosorbent assay kit (Cusabio, CSB-E13462 h, Houston, Texas) according to the manufacturer’s instructions.

    Techniques: Inhibition, Western Blot, Recombinant, Phospho-proteomics, Control

    Extracellular heat shock protein 90α (HSP90α) but not HSP90β increased the phosphorylation levels of Ak strain transforming (AKT) and glycogen synthase kinase 3β (GSK3β) while enhanced the expression of active β-catenin. (A) Tumor volumes of xenograft mouse after various treatments. (B) Representative and (C) summary of the phosphorylation and expression levels in tumor areas. (D) Representative and (E) summary of the transferase dUTP nick end labeling (TUNEL) staining indicating the apoptosis of tumor cells under different treatment. * P < .05, ** P = .01, *** P = .001, and † P > .05, respectively, compared to control. # P < .05, ## P = .01, ### P = .001, and ‡ P > .05, respectively, compared to models or indicated.

    Journal: Cancer Control : Journal of the Moffitt Cancer Center

    Article Title: Secreted Heat Shock Protein 90α Attenuated the Effect of Anticancer Drugs in Small-Cell Lung Cancer Cells Through AKT/GSK3β/β-Catenin Signaling

    doi: 10.1177/1073274818804489

    Figure Lengend Snippet: Extracellular heat shock protein 90α (HSP90α) but not HSP90β increased the phosphorylation levels of Ak strain transforming (AKT) and glycogen synthase kinase 3β (GSK3β) while enhanced the expression of active β-catenin. (A) Tumor volumes of xenograft mouse after various treatments. (B) Representative and (C) summary of the phosphorylation and expression levels in tumor areas. (D) Representative and (E) summary of the transferase dUTP nick end labeling (TUNEL) staining indicating the apoptosis of tumor cells under different treatment. * P < .05, ** P = .01, *** P = .001, and † P > .05, respectively, compared to control. # P < .05, ## P = .01, ### P = .001, and ‡ P > .05, respectively, compared to models or indicated.

    Article Snippet: Heat shock protein 90α levels were detected using Hsp90α enzyme-linked immunosorbent assay kit (Cusabio, CSB-E13462 h, Houston, Texas) according to the manufacturer’s instructions.

    Techniques: Phospho-proteomics, Expressing, End Labeling, TUNEL Assay, Staining, Control